The Brazilian was on the bench for their 3-0 loss to Arsenal in the Premier League at the weekend.
Manager Roberto De Zerbi felt that Pedro was not quite ready to play the whole contest.
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Post-game, De Zerbi said: I made a mistake at Brentford because Joao Pedro couldn't play in the first XI (on Saturday) because I kept him on the pitch for 90 minutes.
In my head before the Brentford game was to change him after 65 minutes.
But to try to win the game and on the bench there wasn't another striker, I made a mistake."