The Dutchman suffered a serious ankle injury during yesterday's 3-2 defeat at Real Madrid.
Barca medical chiefs say De Jong won't play again this season. However, there is hope he will make the Euros for Holland.
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De Jong is set to be sidelined for 5-6 weeks.
Barca announced today: "The tests carried out this Monday have confirmed that the first team player Frenkie De Jong suffers a sprain in his right ankle. It is low and its evolution will mark his availability."