Ancelotti says the fit-again defender will face Real Mallorca.
He said: "I repeat what I said: they are all available and tomorrow I will put in the best team. Militao is also available."
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On the prospect of Militao marking Vedat Muriqi, Ancelotti also said: "It's a thought that we have in our heads and we have to think about it, of course, but der has worked hard and is physically fine.
"If I see a player well, I don't think about the minutes he has spent or the time he accumulates outside. I put (Sami) Khedira, who was coming off an injury, in a Champions League final and I did it because I saw him well, at that moment, that night. Obviously he can't play 90 minutes, that's complicated, but he can last a good part of the game, yes."