The former Napoli and Valencia departs after only months in charge.
"Olympique Marseille announces the end of the collaboration with Gennaro Gattuso," club statement read this morning.
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"Olympique Marseille thanks in particular Gennaro and all his staff for the great professionalism demonstrated on a daily basis and wishes them good luck for the future."
Gattuso was announced as OM's new coach on 27 September: at that time the team was seventh in Ligue 1 with 9 points after six rounds. Now, however, Marseille is ninth in the standings with thirty points won in twenty-two games.