Italy have friendlies against Venezuela and Ecuador later in March.
Spalletti has named talented youngsters Bellanova and Lucca in his 28-man squad set to play in the USA.
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Also earning a recall is Genoa striker Mateo Retegui.
Italy squad:
Goalkeepers: Donnarumma, Vicario, Meret, Carnesecchi.
Defenders: Di Lorenzo, Bellanova, Darmian, Acerbi, Bastoni, Buongiorno, Scalvini, Dimarco, Udogie, Cambiaso.
Midfielders: Barella, Bonaventura, Folorunsho, Frattesi, Jorginho, Locatelli, Pellegrini.
Forwards: Chiesa, Lucca, Orsolini, Raspadori, Retegui, Zaccagni, Zaniolo.