Notably, former Liverpool midfielder and Three Lions staple Jordan Henderson has been dropped.
Manchester United striker Marcus Rashford is another big name left out of Gareth Southgate's training squad.
Crystal Palace quartet Eberechi Eze, Adam Wharton, Marc Guehi and Dean Henderson have made the cut.
Liverpool duo Curtis Jones and Jarrell Quansah have also been selected, alongside Everton defender Jarrad Branthwaite.
Ben Chilwell, who had hoped to deputise for Manchester United left-back Luke Shaw, has not been included.
Manchester City's Kalvin Phillips, on loan at West Ham since January, also misses out.
Burnley goalkeeper James Trafford makes the 33-man squad, but Chelsea's Raheem Sterling doesn't.
England will play friendly matches against Bosnia & Herzegovina and Iceland next month, in the build-up to Euro 2024.
England squad:
Goalkeepers: Dean Henderson (Crystal Palace), Jordan Pickford (Everton), Aaron Ramsdale (Arsenal), James Trafford (Burnley)
Defenders: Jarrad Branthwaite (Everton), Lewis Dunk (Brighton & Hove Albion), Joe Gomez (Liverpool), Marc Guehi (Crystal Palace), Ezri Konsa (Aston Villa), Harry Maguire (Manchester United), Jarell Quansah (Liverpool), Luke Shaw (Manchester United), John Stones (Manchester City), Kieran Trippier (Newcastle United), Kyle Walker (Manchester City)
Midfielders: Trent Alexander-Arnold (Liverpool), Jude Bellingham (Real Madrid), Conor Gallagher (Chelsea), Curtis Jones (Liverpool), Kobbie Mainoo (Manchester United), Declan Rice (Arsenal), Adam Wharton (Crystal Palace)
Forwards: Jarrod Bowen (West Ham United), Eberechi Eze (Crystal Palace), Phil Foden (Manchester City), Anthony Gordon (Newcastle United), Jack Grealish (Manchester City), Harry Kane (Bayern Munich), James Maddison (Tottenham Hotspur), Cole Palmer (Chelsea), Bukayo Saka (Arsenal), Ivan Toney (Brentford), Ollie Watkins (Aston Villa)