Southampton finished their 1-1 draw against Manchester City with four 18 year-olds on the pitch.
Luke Shaw and James Ward-Prowse have been regulars in Mauricio Pochettino's side this season.
Calum Chambers joined them against City at right back after Nathaniel Clyne was ruled out through injury.
And Harrison Reed was brought in with just a few minutes remaining for his Premier League debut.
It is believed to be the first time in Premier League history that a side has fielded four 18-year-olds at the same time, reports the Daily Echo.
